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Equipment for High Throughput Screening (HTS)
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Sagian Core System I Flex Station II: 96 and 384 well plate reader (Molecular Devices): GPCR and ion channel assays and general fluorescence applications. Victor 3V: 96 or 384 and 1536 well : UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay. Biomek FX: Liquid handler for compound transfer and cherry picking. It is equipped with a custom built pin tool and transfers 384 compounds in 85 seconds. ORCA robotic arm on a rail: delivers and transfers plates from plate hotels or incubators onto the liquid handlers, plate readers, etc. Cytomat 6001: CO2 plate incubator with a capacity of 180 plates. Multidrop 384: (Thermo LabSystems): manifold liquid dispensing. This instrument is useful for accurately delivering a fixed amount of liquid rapidly (about 25 seconds) into either 96 or 384 well plates and plating of cells. ELx 405 plate washer, Bio-Tek Instruments: well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing into wells. Lidding station: Automated storage of lids. All devices are integrated under SILAS/SAMI software for walk-away automation. Sagian Core System II Currently under construction: An ORCA based system which is dedicated to high-content screening. Other instruments available: Qbot: Colony picker and arrayer (Genetix). This instrument has a 72 plate capacity and is used in high-throughput sequencing projects as well as for library construction and maintenance. It uses 96 and 384 well plates, omni-trays and is equipped with a video camera for automated colony recognition and blue-white screening. Precision 2000: A 12 channel liquid handler (Bio-Tek) which can be used in a steril bench and adresses 96 and 384 well plates. Various Multidrop 384's: (Thermo LabSystems) This instrument is useful for accurately delivering a fixed amount of liquid rapidly into either 96 or 384 well plates and plating of cells. |
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