Liquid Handlers
Multidrop384 (Thermo LabSystems): manifold liquid dispensing. This instrument is useful for accurately delivering a fixed amount of liquid rapidly (about 25 seconds) into either 96 or 384 well plates and plating of cells.
ELx 405 plate washer (BioTek): well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing into wells
ELx 406 plate washer (BioTek): Dual Syring pump, peristaltic pump, 96, 384 and 1536 well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing of reagents into wells
Echo 555 (Beckman Coulter): Acoustic dispensing system used for 384 to 384 and 1536 to 1536 dispensing of any well to any well in any volume. Mostly used for the generation of primary assay plates and dose response plates, the system is also capable of setting up cloning, qPCR and re-arraying experiments. It is extremely fast and accurate to 2.5 nL volumes.
Aquamax (Molecular Devices): Liquid handling system that integrates four-fluid dispensing and washing for 96, 384, amd 1536 well washing.
Mantis (Formulatrix): Low dead-volume, non-contact liquid dispenser that can up to six reagents that allows dispensing complex plates and assay development experiments down to 100nL volumes.
Precision 2000 (BioTek): A 12 channel liquid handler (Bio-Tek) which can be used in a sterile bench and addresses 96 and 384 well plates.
EL 406 (BioTek): well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing into wells. It is also equiped with dual syringe dispensers as well as a peristaltic pump for reagent addition.
Agilent Bravo (Agilent): Pipettor for 96,384 and 1536 well plates.
Biomek FxP (Beckman): Pipettor for 96,384 and 1536 well plates, useful for cherry picking, re-arraying etc. We have three systems available for use.
Plate Readers
Acumen Explorer(TTP Labtech): This laser scanning cytometer is currently the fastest high content screening system available. It enables cell-by-cell readouts resulting in a flow-cytometer like data structure useful for phenotypic screening. Examples are screens based on translocation events from the nucleus to the cytosol, cytotoxicity screens and even cell cycle based screening is possible.
ImageXpress(Molecular Devices) XL: A high throughput epifluorescence microscope which can be used for phenotypic screens requiring the highest quality images such as neurite outgrowth, translocation and certain cell toxicity screens. We have a phase contrast option enabling label free phenotypic screening of zebrafish. Using special objectives and analysis modules, even difficult phenotypes such as the of uptake of bacteria by mammalian cells can be quantified. This system is integrated with an incubator using a custom Denso robotic arm.
ImageXpress Confocal(Molecular Devices) I and II: A high throughput spinning disk microscope that also doubles as wide field microscope and can be used for phenotypic screens requiring optical sectioning, subcellular localization, mitochondrial morphology evaluation and other demanding applications. We have over 750TB storage capacity integrated and high speed data analysis capabilities as well as custom module editor software to simplify complex analysis workflows. We have specialized water optics that enable imaging or organoids, optical sectioning of 3D structures and live cell imaging at 37°C.
Envision(Perkin Elmer): High speed plate reader for 96 or 384 and 1536 well plates in the following modes: UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay as well as alpha screening. We have two systems available.
Flex Station III(Molecular Devices): 96 and 384 well plate reader. GPCR and ion channel assays and general fluorescence applications.
Victor 3V(Perkin Elmer): 96 or 384 and 1536 well : UV-VIS absorbance, luminescence, fluorescence polarization, luorescence, time resolved fluorescence assay
Other
Biosorter (Union Biometrica): The BioSorter® is a continuous flow system capable of analyzing, sorting and dispensing objects ranging in size from 10 to 1,500 µm. It is useful for sorting zebrafish, C.Elegans, organoids, spheroids and other large objects based on either fluorescence or backscatter/size. Moreover, the MSSR has fitted this biosorter with an in house built aerosol management system that enables us to sort live particles into 96 or even 384 well plates and we can define the number of objects and characteristics of the objects on a per well level enabling screening of large biological objects with defined parameters.
Q-Bot (Genetix): Colony picker and arrayer (Genetix). This instrument has a 72 plate capacity and is used in high-throughput sequencing projects as well as for library construction and maintenance. It uses 96 and 384 well plates, omni-trays and is equipped with a video camera for automated colony recognition and blue-white screening.
BioMek FX (Beckman Coulter): Liquid handler for compound transfer and cherry picking. It is equipped with a custom built pin tool and transfers 384 compounds in 85 seconds.
Thermo Spinnaker System I
Flex Station II: 96 and 384 well plate reader (Molecular Devices): GPCR and ion channel assays and general fluorescence applications.
Envision: 96 or 384 and 1536 well : UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay.
Biomek FX: Liquid handler for compound transfer and cherry picking. It is equipped with a custom built pin tool and transfers 384 compounds in 85 seconds.a
Thermo Spinnaker robotic arm on a rail: delivers and transfers plates from plate hotels or incubators onto the liquid handlers, plate readers, etc.
Liconic STX 220: CO2 plate incubator with a capacity of 220 plates.
Multidrop 384: (Thermo LabSystems): manifold liquid dispensing. This instrument is useful for accurately delivering a fixed amount of liquid rapidly (about 25 seconds) into either 96 or 384 well plates and plating of cells.
ELx 405 plate washer, Bio-Tek Instruments: well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing into wells.
Aquamax DW4: Liquid handling system that integrates four-fluid dispensing and washing for 96, 384, amd 1536 well washing.
Thermo Spinnaker System II
A linear rail based system which is dedicated to high-content screening.
ImageXpress Confocal (Molecular Devices) I: A high throughput spinning disk microscope that also doubles as wide field microscope and can be used for phenotypic screens requiring optical sectioning, subcellular localization, mitochondrial morphology evaluation and other demanding applications.
ImageXpress Confocal (Molecular Devices) II: A customized high throughput spinning disk microscope with phase contrast as well as water objectives. This system has a class IV laser 7-laser illumination source required for optical sectioning, subcellular localization, mitochondrial morphology evaluation and other demanding application in 3D.
We have over 750TB storage capacity integrated and high speed data analysis capabilities as well as custom module editor software to simplify complex analysis workflows.
Envision: High speed plate reader for 96 or 384 and 1536 well plates in the following modes: UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay as well as alpha screening.
Cytomat 6001: CO2 plate incubator with a capacity of over 180 plates.
ELx 406 plate washer, Bio-Tek Instruments: well washing, aspiration, dispensing. This instrument would be useful in performing assays which require multi-step aspiration from and dispensing into wells. It is also equiped with dual syringe dispensers as well as a peristaltic pump for reagent addition.
All devices are integrated under Momentum software for walk-away automation.
NGS library preparation system
- Integrated Highres Bio Star System:
- Staubli TX90 robotic arm
- Highres Cellario Scheduler
- Highres SteriStore incubator
- Highres Microserve
- KBiosystems Wasp plate sealer
- KBiosystems plate piercer
- Beckman Biomek Fxp liquid handler
Compounding and Assay Plate Production System
- Integrated system 3 with F7 arm:
- Xpeel
- Wasp plate sealer
- Liconic -20°C incubator for 220 plates
- Liconic -4°C incubator for 220 plates
- Bravo liquid handler
- Echo 555 acoustic dispenser
- F5 Fanuc robotic arm
- Three automatic PCR systems in 384 well format
- Agilent Vspin
Barcoding and Compound Plate manufacturing system
This system is ideal for plate production of compound library plates or . It consists of a Velocity 11 Benchcell 4R with Vprep, PlateLoc and Vcode and a custom waste shoot. It’s enclosed in a HEPA filtered system for operator protection and is suited for lentiviral arrayed library work.
Other Available Instruments
Biosorter (Union Biometrica): The BioSorter® is a continuous flow system capable of analyzing, sorting and dispensing objects ranging in size from 10 to 1,500 µm. It is useful for sorting zebrafish, C.Elegans, organoids, spheroids and other large objects based on either fluorescence or backscatter/size. Moreover, the MSSR has fitted this biosorter with an in house built aerosol management system that enables us to sort live particles into 96 or even 384 well plates and we can define the number of objects and characteristics of the objects on a per well level enabling screening of large biological objects with defined parameters.
Qbot: Colony picker and arrayer (Genetix). This instrument has a 72 plate capacity and is used in high-throughput sequencing projects as well as for library construction and maintenance. It uses 96 and 384 well plates, omni-trays and is equipped with a video camera for automated colony recognition and blue-white screening.
Precision 2000 (BioTek): A 12 channel liquid handler (Bio-Tek) which can be used in a sterile bench and addresses 96 and 384 well plates.
Various Multidrop 384’s: (Thermo LabSystems) This instrument is useful for accurately delivering a fixed amount of liquid rapidly into either 96 or 384 well plates and plating of cells.
Acumen Explorer (TTP Labtech): This laser scanning cytometer is currently the fastest high content screening system available. It enables cell-by-cell readouts resulting in a flow-cytometer like data structure useful for phenotypic screening. Examples are screens based on translocation events from the nucleus to the cytosol, cytotoxicity screens and even cell cycle based screening is possible.
ImageXpress (Molecular Devices): A high throughput epifluorescence microscope which can be used for phenotypic screens requiring the highest quality images such as neurite outgrowth, translocation and certain cell toxicity screens. We have a phase contrast option enabling label free phenotypic screening of zebrafish. Using special objectives and analysis modules, even difficult phenotypes such as the of uptake of bacteria by mammalian cells can be quantified. This systen is integrated with an incubator using a custom Denso robotic arm.
Mantis(Formulatrix): Low dead-volume, non-contact liquid dispenser that can up to six reagents that allows dispensing complex plates and assay development experiments down to 100nL volumes.